Disease Information

25 November 2005
Vol. 18 - No. 47

Contents
Newcastle disease in France in pigeons: follow-up report No. 1 (confirmation of diagnosis)
Highly pathogenic avian influenza in China (People's Rep. of ~): follow-up report No. 9
Bonamia ostreae in Morocco
Newcastle disease in Romania
Highly pathogenic avian influenza in Romania: follow-up report No. 8
American foulbrood in Chile: follow-up report No. 3
Foot and mouth disease in Russia: virus type Asia1 (follow-up report No. 3 [final report])
American foulbrood in Norway: follow-up report No. 2 (final report)
Equine encephalomyelitis (Eastern) in Belize
Highly pathogenic avian influenza in Thailand: follow-up report No. 77
Miscellaneous: Avian influenza in Canada
Miscellaneous: West Nile fever in Belize

NEWCASTLE DISEASE IN FRANCE IN PIGEONS
Follow-up report No. 1 (confirmation of diagnosis)

See also: 9 November 2006, 12 October 2006, 23 December 2005, 9 December 2005, 18 November 2005, 11 November 2005, 4 November 2005, 28 October 2005, 26 August 2005, 5 August 2005, 29 July 2005, 22 July 2005

Translation of information received on 18 November 2005 from Dr Monique Eloit, Deputy Director General, General Directorate for Food (DGAL), Ministry of Agriculture, Food, Fisheries and Rural Affairs, Paris:

End of previous report period: 8 November 2005 (see Disease Information, 18 [45], 423, dated 11 November 2005).

End of this report period: 18 November 2005.

Date of first confirmation of the event: 7 November 2005.

Date of start of the event: 31 October 2005.

Diagnosis (updated information):

Laboratory where diagnostic tests were performedDiagnostic tests usedDateResults
Côtes-d'Armor Department Laboratory

- haemagglutination inhibition test;

- virus isolation.

7 Nov. 2005positive for APMV1
intracerebral pathogenicity index test17 Nov. 2005ICPI=1.04
AFSSA(1) Ploufragan (national reference laboratory)sequencing*

* Amino acid sequence at the fusion protein cleavage site:

   112   116117  
SGGRRQKRFIG

Source of outbreak: unknown or inconclusive. Contact with wild birds is suspected.

Control measures undertaken:

- stamping out (the remaining 8,200 pigeons in the affected farm were preventively killed on 7 November 2005 and then destroyed);

- quarantine;

- movement control inside the country;

- in accordance with the regulations in force (European Union Council Directive 92/66/EEC), a 3-km-radius protection zone and a 10-km-radius surveillance zone have been set up;

- disinfection of infected premises/establishment.

Vaccination prohibited: no.

Other details/comments:

- There were no movements of birds from this farm to other countries during the 21-day risk period before the onset of the clinical signs (the first clinical signs were observed on 31 October 2005).

- The only species of birds reared on the farm were pigeons for slaughter purposes only.

- The preliminary epidemiological investigation conducted in farms situated within a 3-km radius of the outbreak found no clinical signs of Newcastle disease.

Final report: no.

(1) AFSSA: Agence française de sécurité sanitaire des aliments (French Agency for Food Safety)

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HIGHLY PATHOGENIC AVIAN INFLUENZA IN CHINA (PEOPLE'S REP. OF ~)
Follow-up report No. 9

See also: 5 October 2006, 17 August 2006, 27 July 2006, 6 July 2006, 22 June 2006, 8 June 2006, 9 February 2006, 12 January 2006, 5 January 2006, 16 December 2005, 2 December 2005, 18 November 2005, 11 November 2005, 11 November 2005, 4 November 2005, 28 October 2005, 21 October 2005, 12 August 2005

Information received on 18, 20, 22 and 23 November 2005 from Mr Jia Youling, Director General, Veterinary Bureau, Ministry of Agriculture, Beijing:

End of previous report period: 17 November 2005 (see Disease Information, 18 [46], 435, dated 18 November 2005).

End of this report period: 23 November 2005.

Precise identification of agent: highly pathogenic avian influenza virus subtype H5N1.

Date of first confirmation of the event: 7 June 2005.

Nature of diagnosis: clinical, post-mortem and laboratory.

Details of new outbreaks:

First administrative divisionLower administrative divisionType of epidemiological unitName of the locationDate of start of the outbreakSpeciesNumber of animals in the outbreaks
susceptiblecasesdeathsdestroyedslaughtered
Hubei provinceShishou cityvillageSwan economic developing areaNov. 2005avi7,3003,5003,5003,8000
Inner Mongolia autonomous regionMolidawa Dawo'er autonomous countyvillageWulan15 Nov. 2005avi1761763,2020
Ningxia autonomous regionYinchuan cityvillageShangqian Cheng17 Nov. 2005avi1,1302942309000
Shanxi provinceXiaoyi cityvillageGaoyang10 Nov. 2005avi9,3868,1038,1031,2830
Xinjiang autonomous regionMiquan cityvillageNiuzhuangzi15 Nov. 2005avi6,1202,0642,0644,0560
Xinjiang autonomous regionUrumchi cityvillageDaban Cheng16 Nov. 2005avi1253838870
Xinjiang autonomous regionUrumchi cityvillageWulabo12 Nov. 2005avi5688480
Yunnan provinceChuxiong cityvillageHehua17 Nov. 2005avi11,0002,5002,5008,5000

Description of affected population in the new outbreaks: chickens and geese.

Diagnosis:

Laboratory where diagnostic tests were performedDiagnostic tests usedDateResults
Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin (national reference laboratory for avian influenza)

- haemagglutination inhibition test;

- RT-PCR(1).

16-23 Nov. 2005positive
intravenous pathogenicity index (IVPI) test16-23 Nov. 2005positive (highly pathogenic)

Source of new outbreaks: contact with wild birds.

Control measures undertaken:

- stamping out applied to 254,464 birds around the outbreaks;

- quarantine;

- movement control inside the country;

- screening;

- zoning;

- vaccination;

- disinfection of infected premises/establishments;

- dipping/spraying.

Final report: no.

(1) RT-PCR: reverse transcriptase - polymerase chain reaction

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BONAMIA OSTREAE IN MOROCCO

Immediate notification report and final report

Translation of information received on 21 November 2005 from Dr Hamid Benazzou, Director of Animal Production Department, Ministry of Agriculture and Rural Development, Rabat:

Report date: 15 November 2005.

Reason for immediate notification: first occurrence or re-occurrence in a country or zone /compartment of the country, if the country or zone/compartment of the country was previously considered to be free of that particular disease.

Date of first confirmation of the event: 13 October 2005.

Date of start of the event: 20 June 2005.

Clinical disease: yes.

Nature of diagnosis: basic laboratory tests.

Details of occurrence:

First administrative divisionLower administrative divisionType of epidemiological unitName of the locationSpeciesNumber of animals in the occurrence
susceptiblecasesdeathsdestroyedslaughtered
Laayoune provinceAkhfennir rural districtfarmKhnifiss lagoonmol70058005800

Description of affected population: flat oysters (Ostrea edulis) in closed farming.

Diagnosis:

Laboratories where diagnostic tests were performedDiagnostic tests usedDateResults
Laboratory for Veterinary Tests and Research, Agadirpositive
National Fisheries Research Institute, Casablancapositive
IFREMER(1) (OIE Reference Laboratory for mollusc diseases)positive

Source of occurrence or origin of infection: introduction of new live aquatic animals.

Control measures undertaken: official destruction of clinically diseased aquatic animals.

Treatment of affected animals: no.

Final report: yes.

(1) IFREMER: Institut français de recherche pour l'exploitation de la mer (French Research Institute for Exploitation of the Sea)

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NEWCASTLE DISEASE IN ROMANIA

See also: 21 December 2006, 2 November 2006, 15 June 2006, 6 April 2006, 9 March 2006, 23 February 2006, 2 February 2006, 19 January 2006, 5 January 2006, 30 December 2005, 16 December 2005, 9 December 2005, 2 December 2005

(Date of previous outbreak of Newcastle disease in Romania reported to the OIE: December 1985).

Immediate notification report

Information received on 22 November 2005 from Dr Gabriel Predoi, Director General, National Sanitary Veterinary and Food Safety Authority, Bucharest:

Report date: 22 November 2005.

Reason for immediate notification: re-occurrence of a listed disease or infection in a country or zone/compartment following a report declaring the outbreak(s) ended.

Date of first confirmation of the event: 14 October 2005.

Date of start of the event: 15 September 2005.

Nature of diagnosis: clinical, post-mortem and laboratory.

Details of outbreaks:

First administrative division (County)Lower administrative division (district)Type of epidemiological unitName of the locationDate of start of the outbreakSpeciesNumber of animals in the outbreaks
susceptiblecasesdeathsdestroyedslaughtered
ConstantaSilisteavillageSilistea14 Oct. 2005avi170170*123*470
TulceaSalva RusaIazurile20 Oct. 2005fau1**1**

* hens

** partridge

Description of affected populations:

- The affected hens were an unvaccinated backyard flock.

- The partridge was found dead in the wild and sent to the laboratory by the Sport Association of Hunters and Fishermen, Babadag, Tulcea County.

Diagnosis:

Laboratory where diagnostic tests were performedSamples examinedDiagnostic tests usedDateResults
Institute for Diagnostic and Animal Health (National Reference Laboratory)

- 1 organ sample taken from 1 hen;

- 1 sample of allanto-amniotic fluid taken from eggs inoculated with brain, organs and cloacal swabs taken from hens;

- 1 tracheal swab and 1 cloacal swab taken from 1 partridge.

- intracerebral pathogenity index test on day-old SPF chicks;

- virus isolation in embryonated eggs.

16 Nov. 2005positive

Source of outbreaks or origin of infection: contact with wild birds.

Control measures undertaken:

- stamping out applied to the infected backyard flock;

- quarantine;

- movement control inside the country;

- screening;

- zoning;

- disinfection of infected premises.

Vaccination prohibited: no.

Final report: no.

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HIGHLY PATHOGENIC AVIAN INFLUENZA IN ROMANIA
Follow-up report No. 8

See also: 2 March 2006, 23 February 2006, 16 February 2006, 19 January 2006, 5 January 2006, 30 December 2005, 23 December 2005, 16 December 2005, 9 December 2005, 2 December 2005, 2 December 2005, 18 November 2005, 4 November 2005, 4 November 2005, 28 October 2005, 21 October 2005, 21 October 2005, 14 October 2005, 14 October 2005

Information received on 22 November 2005 from Dr Gabriel Predoi, Director General, National Sanitary Veterinary and Food Safety Authority, Bucharest:

End of previous report period: 16 November 2005 (see Disease Information, 18 [46], 439, dated 18 November 2005).

End of this report period: 22 November 2005.

In accordance with the zoosanitary provisions in force (i.e. Ministry of Agriculture and Food Order No. 156/27.12.1999 regarding the announcing, the declaring and notification of some transmissible diseases of animals, and the ‘Programme for surveillance, prevention and control of diseases of animals and diseases transmissible from animals to humans, and for animal protection and protection of environment for the year 2005’), the three outbreaks of Ceamurlia-de-Jos(1), Maliuc(2) and Vulturu(2), have been eradicated, as follows:

A. Outbreak reported in backyard premises in Ceamurlia-de-Jos, Ceamurlia-de-Jos district, Tulcea County:

The outbreak was declared extinguished after 38 days, since the serological examination of sentinel poultry gave negative results ([Analysis Bulletin No. 16698 - 16985/11.11.2005]). In addition, the sentinel poultry did not manifest any signs of disease suggesting highly pathogenic avian influenza (HPAI).

Three successive disinfections were carried out.

The quarantine measures were lifted on 11 November 2005.

The outbreak was declared extinguished as of 21 November 2005.

B. Outbreak reported in swans in Maliuc, Maliuc district, Tulcea County, and outbreak reported in backyard premises in Vulturu, Maliuc district, Tulcea County:

The outbreaks were declared extinguished after 35 days, since the tests carried out (RT-PCR(3) test for the detection of specific viral genome for HPAI [Analysis Bulletin No. 17941-1800/21.11.2005]) gave negative results for the primary pathological material examined.

The sentinel poultry did not manifest any signs of disease suggesting HPAI.

Three successive disinfections were carried out in both localities.

The quarantine measures were lifted on 21 November 2005 and the outbreaks were declared extinguished.

For the moment, the single active outbreak in Romania is that of Caraorman(4), Tulcea County.

Final report: no.

(1) See Immediate notification report, Follow-up report No. 1 and Follow-up report No. 3

(2) See Follow-up report No. 2 and Follow-up report No. 3

(3) RT-PCR: reverse transcriptase - polymerase chain reaction

(4) See Follow-up report No. 7

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AMERICAN FOULBROOD IN CHILE
Follow-up report No. 3

See also: 30 March 2006, 2 March 2006, 26 January 2006, 12 January 2006, 23 December 2005, 16 December 2005, 9 December 2005, 2 December 2005, 18 November 2005, 4 November 2005, 28 October 2005

Translation of information received on 22 November 2005 from Dr Hernan Rojas Olavarria, Head, Department of Animal Protection, Department of Agriculture and Animal Production (SAG), Ministry of Agriculture, Santiago:

End of previous report period: 11 November 2005 (see Disease Information, 18 [46], 434, dated 18 November 2005).

End of this report period: 22 November 2005.

Precise identification of agent: Paenibacillus larvae subsp. larvae.

Date of first confirmation of the event: 20 October 2005.

Date of start of the event: 15 October 2005.

Nature of diagnosis: clinical and laboratory.

Details of new outbreaks:

First administrative divisionLower administrative divisionsType of epidemiological unitName of the location (sector)LatitudeLongitudeDate of start of the outbreakSpeciesNumber of animals* in the outbreaks
susceptiblecasesdestroyed
V RegionSan Felipe, San Felipe communeapiarySan Felipe32 44´26.50302"S70 49´29.7173"W5 nov. 2005api10042
V RegionSan Felipe, San Felipe communeapiarySan Felipe32 44´58.7708"S70 49´47.4714"W6 nov. 2005api10050
V RegionSan Felipe, San Felipe communeapiaryQuebrada de Herrera32 41´21.3116"S70 44´39.4894"W21 oct. 2005api2930
V RegionSan Felipe, San Felipe communeapiaryQuebrada de Herrera32 41´19.5113"S70 44´49.8591"W20 oct. 2005api6511
VII RegionCuricó, Curicó communeapiaryHuañuñe35 2´24.2391"S71 9´56.9466"W5 nov. 2005api4811
VII RegionTalca, Pencahue communeapiaryLos Litres35 14´33.5650"S71 36´52,7423" W9 nov. 2005api2801717

* hives

Diagnosis:

Laboratory where diagnostic tests were performedDiagnostic tests usedDateResults
Department of Laboratories and Plant and Animal Quarantine Stations, Lo Aguirre, Santiago de Chile (official SAG laboratory)PCR(1)15-17 Nov. 2005positive

Source of new outbreaks: contact with affected hives.

Control measures undertaken:

- quarantine;

- partial stamping out;

- movement control inside the country.

- zoning.

Final report: no.

(1) PCR: polymerase chain reaction

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FOOT AND MOUTH DISEASE IN RUSSIA
Virus type Asia1 (follow-up report No. 3 [final report])

See also: 26 January 2006, 23 December 2005, 16 September 2005

Information received on 23 November 2005 from Dr Evgueny A. Nepoklonov, Head of the Main Veterinary Department, Ministry of Agriculture and Food, Moscow:

End of previous report period: 7 September 2005 (see Disease Information, 18 [37], 301, dated 16 September 2005).

End of this report period: 21 November 2005.

Date of first confirmation of the event: 12 June 2005.

Date of start of the event: 6 June 2005.

No new outbreaks of foot and mouth disease (FMD) due to virus serotype Asia1 have been detected since 10 September 2005.

By 15 October 2005, quarantine had been lifted in all affected locations (see details in the table below).

Details of outbreaks (updated information):

First administrative divisionLower administrative division (district)Type of epidemiological unitName of the locationLifting of quarantineSpeciesNumber of animals in the outbreaks
susceptiblecasesdeathsdestroyedslaughtered
Amur region (Amurskaya oblast')SvobodnyyvillageBusse11 July 2005bov1714201710
o/c3700370
sui90090
Khabarovsk TerritoryBikinefarmvillage of Dobrolyubovo29 Sept. 2005bov1067101060
Khabarovsk TerritoryBikinefarmvillage of Lonchakovo29 Sept. 2005bov32320320
Khabarovsk TerritoryViazemskyvillageVidnoye14 Oct. 2005bov20180200
Khabarovsk TerritoryViazemskyfarmvillage of Sheremet’yevo27 Sept. 2005bov56560560
Primorskiy TerritoryKhankavillagePervomaisky12 Oct. 2005bov95320950
o/c3700370
Primorskiy TerritoryKhassanvillageSlavyanka6 Oct. 2005bov12100120
Primorskiy TerritoryKhorolvillageLuchki10 Oct. 2005bov1063611050
ovi10010
Primorskiy TerritoryKhorolfarmvillage of Sivakovka10 Oct. 2005bov33531803350
sui11010
Primorskiy TerritoryKirovskyvillagePavlo-Fedorovka27 Sept. 2005bov32632603260
Primorskiy TerritoryLesozavodskfarmvillage of Nevskoye7 Oct. 2005bov2808202800
Primorskiy TerritoryMikhailovkavillageAbramovka6 Oct. 2005bov2147002140
o/c3900390
Primorskiy TerritoryPozharskoyevillageIgnat’yevka29 Sept. 2005bov1135301130
Primorskiy TerritorySpasskvillageKrasny Kut29 Sept. 2005bov1427301420
o/c1400140
sui10010

Source of outbreaks or origin of infection: unknown or inconclusive.

Control measures applied:

- stamping out;

- quarantine;

- movement control inside the country;

- screening;

- zoning;

- vaccination (see details below);

- disinfection.

Vaccination in response to the outbreaks:

First administrative divisionSpeciesTotal number of animals
vaccinatedrevaccinatedDetails of the vaccine
Khabarovsk Territorybov42,53042,389inactivated sorbat FMD vaccine O, A, Asia1
o/c7,3577,333inactivated sorbat FMD vaccine O, A, Asia1
sui38,83529,754inactivated emulsion FMD vaccine Asia1
Primorsky Territorybov100,82499,105inactivated sorbat vaccine O, A, Asia1
o/c35,11234,456inactivated sorbat vaccine O, A, Asia1
sui56,06338,594inactivated emulsion FMD vaccine Asia1

Note: Before the outbreaks, animals were not vaccinated against FMD of Asia1 serotype.

Final report: yes.

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AMERICAN FOULBROOD IN NORWAY
Follow-up report No. 2 (final report)

See also: 21 October 2005, 14 October 2005

Information received on 24 November 2005 from Dr Keren Bar-Yaacov, Chief Veterinary Officer, Norwegian Food Safety Authority, Brumunddal:

End of previous report period: 18 October 2005 (see Disease Information, 18 [42], 363, dated 21 October 2005).

End of this report period: 24 November 2005.

Precise identification of agent: Paenibacillus larvae subsp. larvae.

Date of first confirmation of the event: 29 September 2005.

Date of start of the event: 12 September 2005.

All infected hives have been destroyed. No new outbreaks have been reported.

Final report: yes.

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EQUINE ENCEPHALOMYELITIS (EASTERN) IN BELIZE

(Date of previous outbreak of Eastern equine encephalomyelitis in Belize reported to the OIE: 1997).

Immediate notification report

Information received on 24 November 2005 from Dr Victor Gongora, Director of Animal Health, Ministry of Agriculture and Fisheries, Belmopan:

Report date: 24 November 2005.

Reason for immediate notification: re-occurrence of a listed disease or infection in a country or zone/compartment following a report declaring the outbreak(s) ended.

Date of first confirmation of the event: 18 November 2005.

Date of start of the event: 18 October 2005.

Nature of diagnosis: clinical and laboratory.

Details of outbreak:

First administrative divisionLower administrative divisionType of epidemiological unitLatitudeLongitudeSpeciesNumber of animals in the outbreak
susceptiblecasesdeathsdestroyedslaughtered
Cayo districtTeakettlevillage1905964 N16304687 Eequ21000

Description of affected population: a four-year old mare that has never been vaccinated against equine encephalomyelitis.

Diagnosis:

Laboratory where diagnostic tests were performedDiagnostic tests usedDateResults
National Veterinary Services Laboratories (NVSL), Ames, Iowa, United States of AmericaIgM capture ELISA(1)18 Nov. 2005positive (> 1:400)

Note: the mare tested negative to West Nile fever and Venezuelan equine encephalomyelitis on IgM capture ELISA.

Source of outbreak or origin of infection: unknown or inconclusive.

Control measures

A. Undertaken:

- control of arthropods;

- screening.

B. To be undertaken:

- vaccination.

Treatment of affected animal: no.

Other details/comments:

- This is the first reported case since 1997, when Eastern equine encephalomyelitis was diagnosed in a single horse in a nearby area.

- Given the recent cases of dengue fever in humans, the Ministry of Health has been spraying to control the mosquito population and screening for dengue throughout Belize, particularly in Cayo district.

- Triple vaccination for Eastern, Western and Venezuelan equine encephalomyelitis is recommended for all horses in Belize.

Final report: no.

(1) ELISA: enzyme-linked immunosorbent assay

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HIGHLY PATHOGENIC AVIAN INFLUENZA IN THAILAND
Follow-up report No. 77

See also: 16 November 2006, 3 August 2006, 27 July 2006, 6 April 2006, 16 February 2006, 2 February 2006, 26 January 2006, 19 January 2006, 12 January 2006, 23 December 2005, 16 December 2005, 9 December 2005, 2 December 2005, 18 November 2005, 4 November 2005, 28 October 2005, 21 October 2005, 14 October 2005, 7 October 2005, 30 September 2005, 23 September 2005, 16 September 2005, 9 September 2005, 2 September 2005, 26 August 2005, 19 August 2005, 12 August 2005, 29 July 2005, 22 July 2005, 15 July 2005

Information received on 25 November 2005 from Dr Yukol Limlamthong, Director General, Department of Livestock Development (DLD), Ministry of Agriculture and Cooperatives, Bangkok:

End of previous report period: 17 November 2005 (see Disease Information, 18 [46], 436, dated 18 November 2005).

End of this report period: 24 November 2005.

No new outbreaks of highly pathogenic avian influenza were reported during the week under report.

Final report: no.

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MISCELLANEOUS: AVIAN INFLUENZA IN CANADA

See also: 4 May 2006, 16 December 2005, 9 December 2005, 2 December 2005

Immediate notification report

Information received on 19 and 20 November 2005 from Dr Brian Evans, Executive Director, Canadian Food Inspection Agency, Ottawa:

Report date: 20 November 2005.

Reason for immediate notification: detection of an H5 subtype of avian influenza in a single domestic duck, reported as per Terrestrial Animal Health Code recommendations*.

Precise identification of agent: influenza virus type A H5 subtype, low pathogenicity, North-American strain; N subtype pending.

Date of first confirmation of the event: 18 November 2005.

Date of start of the event: 17 November 2005.

Clinical disease: no.

Nature of diagnosis: laboratory.

Details of outbreak:

First administrative division (province)Lower administrative division (district)Type of epidemiological unitSpeciesNumber of animals in the outbreak
susceptiblecasesdeathsdestroyedslaughtered
British ColumbiaChiliwackfarmavi55,000

Description of affected population: meat ducks.

The suspect bird was a 40-day-old meat duck collected at processing. The bird was in excellent body condition with submitting criteria of dermatitis. No other visible lesions and no indication of any active disease process were observed on post-mortem examination.

Diagnosis:

Laboratories where diagnostic tests were performedDiagnostic tests usedDateResults
Provincial Agriculture

- PCR(1) (matrix);

- real-time PCR;

- conventional PCR thermocycling (H5).

18 Nov. 2005positive
National Centre for Foreign Animal Disease (NCFAD), Winnipeg(H5)RT-PCR(2) on cloacal swab and allantoic fluids.20 Nov. 2005positive for influenza virus type A subtype H5
RNA molecular analysis20 Nov. 2005low pathogenic North-American sequence

- N type molecular diagnostic as well as conventional methods;

- haemagglutination and haemagglutination inhibition on allantoic fluids.

pendingpending

Source of outbreak or origin of infection: under investigation. Contact with migratory water fowl cannot be excluded.

Control measures undertaken:

- quarantine of the source farm and the owner’s other operations;

- establishment of a 5-km surveillance zone around the source farm;

- voluntary lock down and movement controls on all commercial units within the 5-km zone pending application of formal quarantine;

- sampling of the source farm;

- culling of birds in the source farm;

- sampling of commercial poultry operations in the 5-km zone;

- sampling of owner’s other operations;

- suspension of export certification from the 5-km zone.

Vaccination prohibited: no.

Other details/comments:

Results of sampling conducted in the Province of British Columbia under a national migratory waterfowl sampling and surveillance programme for avian influenza has demonstrated the presence of H5N2 and H5N9 subtypes, all of which have been determined to be low pathogenic and consistent with historically reported North American strains with significant structural differences from the Asian strain of current international animal and human health concern.

Final report: no.

(1) PCR: polymerase chain reaction

(2) RT-PCR: reverse transcriptase - polymerase chain reaction

* Note by the OIE Animal Health Information Department: this disease is included in the new OIE list of diseases approved in May 2005 and due to come into force in January 2006.

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MISCELLANEOUS: WEST NILE FEVER IN BELIZE

Immediate notification report

Information received on 24 November 2005 from Dr Victor Gongora, Director of Animal Health, Ministry of Agriculture and Fisheries, Belmopan:

Report date: 24 November 2005.

Reason for immediate notification: re-occurrence of a listed disease or infection* in a country or zone/compartment following a report declaring the outbreak(s) ended.

Date of first confirmation of the event: 18 November 2005.

Date of start of the event: 22 August 2005.

Nature of diagnosis: clinical and laboratory.

Details of outbreak:

First administrative divisionLower administrative divisionType of epidemiological unitLatitudeLongitudeSpeciesNumber of animals in the outbreak
susceptiblecasesdeathsdestroyedslaughtered
Belize districtCrooked Treevillage965066337306equ91000

Description of affected population: the affected animal is an unvaccinated 9-year-old gelding.

Diagnosis: the affected horse was seen with ataxia and lip smacking on 22 August 2005 but showed no nervous signs when examined by BAHA(1) veterinarians.

Laboratory where diagnostic tests were performedDiagnostic tests usedDateResults
National Veterinary Services Laboratories (NVSL), Ames, Iowa, United States of AmericaIgM capture ELISA(2)18 Nov. 2005positive (> 1:400)

Source of outbreak or origin of infection: contact with wild birds.

Control measures

A. Undertaken:

- control of arthropods;

- screening.

B. To be undertaken:

- vaccination.

Treatment of affected animal: no.

Other details/comments:

- The affected horse suffered a short-lived illness and recovered uneventfully.

- The affected village is in a popular bird sanctuary area where migrant birds are commonly seen.

- Although horses have owners, they roam freely in the community.

- Although vaccine is available in Belize, no horses in this community have ever been vaccinated.

- No more cases were seen in the community.

- Given the recent cases of dengue fever in humans, the Ministry of Health has been spraying to control mosquito populations throughout Belize.

Final report: no.

(1) BAHA: Belize Agricultural Health Authority

(2) ELISA: enzyme-linked immunosorbent assay

* Note by the OIE Animal Health Information Department: this disease is included in the new OIE list of diseases approved in May 2005 and due to come into force in January 2006.

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