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OIE-approved International Standard Reagents

The OIE Biological Standards Commission coordinates a programme for the preparation, validation and distribution of OIE-approved International Standard Reagents for diagnostic assays for infectious diseases of animals. The standards are prepared by an OIE Reference Laboratory in accordance with Guidelines for antibody, antigen and polymerase chain reaction (PCR) standards drawn-up by the Commission in collaboration with other laboratories. Such standard preparations are designated by the OIE as primary reference standards for use in conjunction with tests described in the OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals (mammals, birds and bees). The aim of the programme is to harmonise diagnostic testing and encourage the mutual recognition of test results for international trade.

Currently available OIE-approved International Standard Reagents



Available from

African horse sickness

Enzyme-linked immunosorbent assay

Marisa Arias
Centro de Investigación en Sanidad Animal, Instituto Nacional de Investigación y Tecnología Agraria y Alimentarie (CISA-INIA), 28130 Valdeolmos, Madrid, Spain
Tel: (34-91) 620.23.00
Fax: (34-91) 620.22.47 /

Aujeszky's disease

Enzyme-linked immunosorbent assay;
Virus neutralisation

Dr Marie-Frédérique Le Potier
Anses Ploufragan, Laboratoire de Ploufragan/Plouzané
Unité de Virologie Immunologie Porcines
22440 Ploufragan, France
Tel: +33 (0)2 96 01 62 90
Fax: +33 (0)2 96 01 62 94


Enzyme-linked immunosorbent assay;
Agar gel immunodiffusion

Dr Tracy Sturgill
National Veterinary Services Laboratories, Animal & Plant Health Inspection Service, USDA, P.O. Box 844, 1920 Dayton Avenue, Ames, Iowa 50010, United States of America
Tel: (1-515) 337.79.29
Fax: (1-515) 337.73.97

Bovine brucellosis

Indirect and Competitive enzyme-linked immunosorbent assay; Complement fixation; Agglutination; Buffered Brucella antigen tests; Fluorescence polarisation assay

Dr Adrian Whatmore
Animal and Plant Health Agency
New Haw, Addlestone, Surrey KT15 3NB
United Kingdom
Tel: (+44-1932) 35.76.10  

Fax: (+44-1932) 35.72.16

Brucella abortus

Complement fixation; Agglutination; Buffered Brucella antigen tests; Fluorescence polarisation assay; Indirect and Competitive enzyme-linked immunosorbent assay

Dr Adrian Whatmore
(as above)

Brucella ovis

Complement fixation

Dr Adrian Whatmore
(as above)

Classical swine fever

Virus neutralisation

Dr Helen Crooke
Animal and Plant Health Agency,
New Haw, Addlestone, Surrey KT15 3NB, United Kingdom
Tel: (44-208) 026 9665

Contagious bovine pleuropneumonia

Both irradiated and non-irradiated sera are available. Please contact the Reference Laboratory for advice on their suitability for different types of serological test (complement fixation test, indirect or competitive enzyme-linked immunosorbent assay)

Dr Massimo Scacchia
CESME, Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise 'G. Caporale', Via Campo Boario, 64100 Teramo, Italy
Tel: (39-0861) 33.24.05
Fax: (39-0861) 33.22.51 

Contagious bovine pleuropneumonia

Complement fixation test

Dr Ana Rosa Pombo Botelho
Laboratório Nacional de Investigaçâo Veterinária, Estrada de Benfica 701
1549-011 Lisboa, Portugal
Tel: (+351-21) 711 53 33 / 39 / 40 
Fax: (+351-21) 711 52 36

Enzootic bovine leukosis

Agar gel immunodiffusion;
Enzyme-linked immunosorbent assay

Prof. Thomas Vahlenkamp
Institute of Virology, Centre for Infectious Diseases, Faculty of Veterinary Medicine
Leipzig University, An den Tierkliniken 29
04103 Leipzig, Germany
Tel: (49-341) 97.38.201
Fax: (49-341) 97.38.219

Equine infectious anaemia

Agar gel immunodiffusion

Dr Stephan Zientara
UMR 1161 Virologie Afssa/INRA/ENVA, 23 avenue du Général de Gaulle,
94703 Maisosn-Alfort, France
Tel: 33 (0)1
Fax: 33 (0)1

Equine influenza

Haemagglutination inhibition; single radial haemolysis

Dr Marie-Emmanuelle Behr-Gross
European Directorate for the
Quality of Medicines, Council of Europe,
7 allée Kastner, F-67081 Strasbourg
Cédex, France
Tel: 33 (0)3;
Fax: 33(0)3.;

Equine influenza

Antigens and antisera for
standardisation of vaccines

NIBSC (National Institute for Biological Standards and Control), P.O. Box 1193, Blanche Lane, South Mimms, Potters Bar, Hertfordshire EN6 3QH, United Kingdom
Tel: (44-1707) 65.47.53
Fax: (44-1707) 64.67.30

Equine viral arteritis

Virus neutralisation

Dr Peter J. Timoney
Maxwell H. Gluck Equine Research Center, Dept of Veterinary Science, University of Kentucky, Lexington, Kentucky 40546-0099, United States of America
Tel: (1-859)  47 57 ext. 81094 ;
Fax: (1-859) 257.85.42;

Foot and mouth disease

Enzyme-linked immunosorbent assay (antigen and antibody detection);
Virus neutralisation

Dr Donald King
The Pirbright Institute, Ash Road,
Pirbright, Woking, Surrey, GU24 0NF, United Kingdom
Tel: (44-1483) 23.24.41
Fax: (44-1483) 23.24.48

Foot and mouth disease

Nonstructural protein tests

Dr Edviges Maristela Pituco
Centro Panamericano de Fiebre Aftosa OPS/OMS, Av. President Kennedy 7778, Sao Bento, Duque de Caxias, ZC 25040-004 Rio de Janeiro, Brazil
Tel: (55-21)
Fax: (55-21)

Infectious bovine rhinotracheitis

Virus neutralisation;
Enzyme-linked immunosorbent assay

Dr Eric Dubois
Anses Sophia-Antipolis, Laboratoire d'Etudes et de Recherches
sur les Ruminants et les Abeilles,
105 routes des Chappes, B.P. 111,
06902 Sophia-Antipolis, France
Tel: 33 (0)4
Fax: 33 (0)4

Dr Eefke Weesendorp
Central Veterinary Institute, part of Wageningen UR, Department of Epidemiology, Crisis Organization and Diagnostics (ECD), P.O. Box 65, 8200 AB Lelystad, The Netherlands
Tel : (31-320) 23.81.02
Fax : (31-320) 23.81.53

Ovine and caprine brucellosis (anti-Brucella melitensis

Indirect and Competitive enzyme-linked immunosorbent assay; Fluorescence polarisation assay; Buffered Brucella antigen tests

Dr Adrian Whatmore
(as above

Peste des petits ruminants

Enzyme-linked immunosorbent assay

Dr Genevieve Libeau
CIRAD/EMVT, Campus international de Baillarguet, Montferriez-sur-Lez, B.P. 5035, 34032 Montpellier Cedex 01, France
Tel: 33 (0)4  67 59 37 98
Fax: 33 (0)4


Fluorescent antibody virus neutralisation

Dr Florence Cliquet

Anses Nancy, Laboratorie de la rage et de la faune sauvage, Technopôle Agricole et Vétérinaire - Bâtiment H, CS 40009, 54220 Malzéville, France
Tel: 33 (0)3
Fax: 33 (0)3

Swine vesicular disease

Enzyme-linked immunosorbent assay (antibody detection);

Virus neutralisation

Dr Donald King
The Pirbright Institute, Ash Road,
Pirbright, Woking, Surrey, GU24 0NF, United Kingdom
Tel: (44-1483) 23.24.41
Fax: (44-1483) 23.24.48


Enzyme-linked immunosorbent assay

Dr Maria Angeles Gomez Morales
Istituto Superiore di Sanita, Laboratorio di Parasitoligia, Viale Regina Elena 299, 00161 Roma, Italy
Tel: +390-6



The term 'International Standard Reagent' is synonymous with primary standard. It represents the standard by which all others (secondary standards) are compared and calibrated. Secondary standards may represent national standards or working standards that are in routine use at the diagnostic laboratory level. The secondary standard and not the International Standard are to be used on a daily basis to standardise testing. The International Standard Reagents listed above may be obtained, in small quantities, at the corresponding address. Some laboratories may make a charge for this service.

Should you contact one of these OIE Reference Laboratories to obtain reference materials and get no response, please inform the OIE Scientific Dept.

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