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Reference reagents
WOAH-approved International Standard Reagents
The WOAH Biological Standards Commission coordinates a programme for the preparation, validation and distribution of WOAH-approved International Standard Reagents for diagnostic assays for infectious diseases of animals. The standards are prepared by a WOAH Reference Laboratory in accordance with Guidelines for antibody, antigen and polymerase chain reaction (PCR) standards drawn-up by the Commission in collaboration with other laboratories. Such standard preparations are designated by WOAH as primary reference standards for use in conjunction with tests described in the WOAH Manual of Diagnostic Tests and Vaccines for Terrestrial Animals (mammals, birds and bees). The aim of the programme is to harmonise diagnostic testing and encourage the mutual recognition of test results for international trade.
Currently available WOAH-approved International Standard Reagents
Disease | Test | Available from |
African horse sickness | Enzyme-linked immunosorbent assay | Marisa Arias |
Aujeszky’s disease | Enzyme-linked immunosorbent assay; | Dr Marie-Frédérique Le Potier |
Bluetongue | Enzyme-linked immunosorbent assay; | To be decided https://www.aphis.usda.gov/aphis/ourfocus/animalhealth/lab-info-services/sa_reagents/ct_reagents |
Bovine brucellosis | Indirect and Competitive enzyme-linked immunosorbent assay; Complement fixation; Agglutination; Buffered Brucella antigen tests; Fluorescence polarisation assay | Dr Adrian Whatmore Fax: (+44-1932) 35.72.16 |
Brucella abortus | Complement fixation; Agglutination; Buffered Brucella antigen tests; Fluorescence polarisation assay; Indirect and Competitive enzyme-linked immunosorbent assay | Dr Adrian Whatmore |
Brucella ovis | Complement fixation | Dr Adrian Whatmore |
Classical swine fever | Virus neutralisation | Dr Helen Crooke |
Contagious bovine pleuropneumonia | Both irradiated and non-irradiated sera are available. Please contact the Reference Laboratory for advice on their suitability for different types of serological test (complement fixation test, indirect or competitive enzyme-linked immunosorbent assay) | Dr Massimo Scacchia |
Contagious bovine pleuropneumonia | Complement fixation test | Dr Ana Rosa Pombo Botelho |
Enzootic bovine leukosis | Agar gel immunodiffusion; | Prof. Thomas Vahlenkamp |
Equine infectious anaemia | Agar gel immunodiffusion | Dr Stephan Zientara |
Equine influenza | Haemagglutination inhibition; single radial haemolysis | Dr Marie-Emmanuelle Behr-Gross |
Equine influenza | Antigens and antisera for | NIBSC (National Institute for Biological Standards and Control), P.O. Box 1193, Blanche Lane, South Mimms, Potters Bar, Hertfordshire EN6 3QH, United Kingdom |
Equine viral arteritis | Virus neutralisation | Dr Peter J. Timoney |
Foot and mouth disease | Enzyme-linked immunosorbent assay (antigen and antibody detection); | Dr Donald King |
Foot and mouth disease | Nonstructural protein tests | Dr Edviges Maristela Pituco |
Infectious bovine rhinotracheitis | Virus neutralisation; | Dr Eric Dubois Dr Eefke Weesendorp |
Ovine and caprine brucellosis (anti-Brucella melitensis [ISaBmS]) | Indirect and Competitive enzyme-linked immunosorbent assay; Fluorescence polarisation assay; Buffered Brucella antigen tests | Dr Adrian Whatmore (as above |
Peste des petits ruminants | Enzyme-linked immunosorbent assay | Dr Genevieve Libeau |
Rabies | Fluorescent antibody virus neutralisation | Dr Florence Cliquet Anses Nancy, Laboratorie de la rage et de la faune sauvage, Technopôle Agricole et Vétérinaire – Bâtiment H, CS 40009, 54220 Malzéville, France |
Swine vesicular disease | Enzyme-linked immunosorbent assay (antibody detection); Virus neutralisation | Dr Donald King |
Trichinellosis | Enzyme-linked immunosorbent assay | Dr Maria Angeles Gomez Morales |
The term ‘International Standard Reagent’ is synonymous with primary standard. It represents the standard by which all others (secondary standards) are compared and calibrated. Secondary standards may represent national standards or working standards that are in routine use at the diagnostic laboratory level. The secondary standard and not the International Standard are to be used on a daily basis to standardise testing. The International Standard Reagents listed above may be obtained, in small quantities, at the corresponding address. Some laboratories may make a charge for this service.
Should you contact one of these WOAH Reference Laboratories to obtain reference materials and get no response, please inform the WOAH Scientific Dept.
Contact : [email protected]